Complete ¹H NMR Assignment of Diosgenin Benzoate

Diosgenin (1a) is used as a raw starting material for the synthesis of steroid hormones and contraceptives probably one most commercially important steroidal sapogenins. The literature on 1a extensive, topics range from its isolation variety natural sources to chemical modifications therapeutic properties an anti-cancer, anti-diabetic, anti-thrombosis, anti-inflammatory, anti-viral agent, among others. 1 spectroscopic characteristics have been analyzed long time, but assignment complete H NMR profile remains incomplete due overlapping signals obtained with common spectrometers. In current study, 750-MHz spectrum diosgenin benzoate (1b) has completely assigned by means QM-based iterative full spin analysis (HiFSA), which description intricate products quality control pharmaceuticals. 2 was facilitated taking advantage some shifts described coupling constants cholestane skeleton cholesteryl benzoate, were also recently calculated HiFSA. 3